전류의 양에 따른 백서의 대퇴골 골형성 변화에 관한 연구
AN EXPERIMENTAL STUDY ON THE OSTEOGENESIS INDUCED BY THE AMOUNT OF CONSTANT DIRECT CURRENT IN RAT FEMUR
김동규, 이진용,
소속 상세정보
김동규 ( ) - 경희대학교
이진용 ( ) - 경희대학교 치과대학 소아치과학교실
KMID : 0358919910180010075
Abstract
결론
저자는 전류의 양이 백서의 대퇴골 골형성에 미치는 영향을 관찰하기 위하여 생후 3개월
된 체중 200gm 내외의 웅성백서 18마리를 대조군 3마리, 실험군 15마리로 분류하였다. 대조
군은 전원에 연결하지 않은 dummy electrode를 우측 대퇴골 중앙부에 장착하였으며, 실험
군은 동일 부위에 stainless steel wire로 된 음전극을 장착시키고, 척추와 양측의 장골극을
연결하는 선이 만나는 지점에 양전극을 피하매식하여 전원에 연결한 후 1군에서는 5∼15μ
A, 2군에서는 20∼30μA, 3군에서는 40∼50μA의 직류전기를 각 군당 5마리씩 7일간 적용
하였다. 7일 경과후 모든 동물을 희생시켜 광학현미경과 주사전자현미경으로 관찰하여 다음
과 같은 결론을 얻었다.
1. 대조군과 실험군 공히 음전극부 양측의 골 외면에 막내골화로 골형성이 야기되었으며
실험부측 신생골연에는 골흡수대가 관찰되었고, 음전극 직하방부위의 골내측으로 골증식이
야기되었으며 신생골과 기존골 사이에는 골흡수가 진행되었다.
2. 적용된 전류량의 증가에 따라 골형성은 보다 거칠고 불규칙한 양상으로 현저하게 진행
되었다.
3. 신생골 형성시 최적의 전류범위는 5∼30μA이며, 40∼50μA에서는 조직변성 및 골흡수
가 유발되었다.
4. 골외면에 형성된 신생골의 골소주는 전류량의 증가에 따라 골장축에 보다 직각방향으로
형성되었다.
#초록#
The present study was undertaken to determine the optimum current range which can
elicit an oateogenic response by observing the changes in osteogenesis induced when
varying amount of constant direct current was applied to rat (Sprague-Dawley strain)
femur.
In order to carry out this experiment, 18 rats weighing about 200gm were selected and
divided into 2 groups, one control group consisting of 3 rats and one experimental group
consisting of 15 rats. The control group was received the dummy electrodes that were
not connected to the power supply pack. The experimental group was subdivided into 3
groups according to the amount of constant direct current applied (5-15μA, 20-30μA,
and 40-50μA).
Under the general anesthesia with intravenous injection of thiopental sodium, all
surgery was performed with aseptic precautions, and each animal was administered
soluble cephradine by intramuscular injection at the end of the surgical procedure.
A 0.010 inch stainless steel wire covered with polyethylene tube was tied at the right
mid femoral portion of the rat, and this wire, serving as a cathode, was connected to
the Constant Current Sink. Anode was implanted subcutaneously over the dorsum at the
cross section area where the vertebral column and a line connecting the both iliac
crests, and then connected to the Sink.
All animals sacrificed at 7 days after application of electrical stimulation. 2 sites near
the cathode were prepared for histologic findings including LM and SEM.
Based on the present study the following conclusions can be made:
1. In both control and experimental group, new bone formed by a process of
intramembranous bone formation was appeared in the periosteal and endosteal surface of
the bone at the vicinity of the cathode, and the area of bone resorption was found at
the margin of the newly formed bone, and bone resorption was occurred between newly
formed bone and old bone at the endosteal surface.
2. According to the increased amount of the direct current applied, a new bone
formation was significantly occurred in a more rough and irregular pattern.
3. The optimum current range seemed to be in 5-30 μA and the current in 40-50μA
range caused tissue degeneration and severe bone resorption.
4. In the newly formed bone on the periosteal surface, the new bone trabeculae were
formed at a more perpendicular direction to the long axis of the femur according to the
increased amount of the direct current.
키워드
원문 및 링크아웃 정보
등재저널 정보